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KMID : 0545120000100040539
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 4 p.539 ~ p.543
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Isolation and Characterization of Lacticin 10790,a New Bacteriocin Produced by Lactococcus lactis subsp.cremoris KFCC 10790
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Joo, Nam Eok
Kim, Il Han/Yoo, Jin Young/Lee, Yong Eok
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Abstract
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A new bacteriocin, named lacticin 10790, was purified from Lactococcus lactis subsp. cremoris KFCC 10790 by sequential adsorption, immobilized metal-affinity, cationexchange, and C_18 reverse-phase chromatographies. The molecular mass of the bacteriocin was estimated to be between 3,000 and 3,500 Da. Lacticin 10790 showed a broad antimicrobial spectrum against many gram-positive bacteria. The bacteriocin was stable to heat and in the pH range between 2 and 6. Lacticin 10790 was destroyed by digestion with proteases and exhibited a bactericidal mode of action. An amino acid composition analysis of purified lacticin 10790 revealed a high concentration of hydrophobic amino acids. The N terminus of the bacteriocin was found to be blocked, upon analysis by Edman degradation. The results suggest that lacticin 10790 is a class I bacteriocin.
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